Katalog GES



Gene Coexpression Analysis Reveals Complex Metabolism of the Monoterpene Alcohol Linalool in Arabidopsis Flowers

The cytochrome P450 family encompasses the largest family of enzymes in plant metabolism, and the functions of many of its members in Arabidopsis thaliana are still unknown. Gene coexpression analysis pointed to two P450s that were coexpressed with two monoterpene synthases in flowers and were... Full description

1st Person: Ginglinger, Jean-François verfasserin
Additional Persons: Boachon, Benoit verfasserin; Höfer, René verfasserin; Paetz, Christian verfasserin; Köllner, Tobias G. verfasserin; Miesch, Laurence verfasserin; Lugan, Raphael verfasserin; Baltenweck, Raymonde verfasserin; Mutterer, Jérôme verfasserin; Ullmann, Pascaline verfasserin; Beran, Franziska verfasserin; Claudel, Patricia verfasserin; Verstappen, Francel verfasserin; Fischer, Marc J.C. verfasserin; Karst, Francis verfasserin; Bouwmeester, Harro verfasserin; Miesch, Michel verfasserin; Schneider, Bernd verfasserin; Gershenzon, Jonathan verfasserin; Ehlting, Jürgen verfasserin; Werck-Reichhart, Danièle verfasserin
Source: in Plant Cell Vol. 25, No. 11 (2013), p. 4640-4657
More Articles
Type of Publication: Article
Language: English
Published: 2013
Online: Volltext
  Search for full text
Summary: The cytochrome P450 family encompasses the largest family of enzymes in plant metabolism, and the functions of many of its members in Arabidopsis thaliana are still unknown. Gene coexpression analysis pointed to two P450s that were coexpressed with two monoterpene synthases in flowers and were thus predicted to be involved in monoterpenoid metabolism. We show that all four selected genes, the two terpene synthases (TPS10 and TPS14) and the two cytochrome P450s (CYP71B31 and CYP76C3), are simultaneously expressed at anthesis, mainly in upper anther filaments and in petals. Upon transient expression in Nicotiana benthamiana, the TPS enzymes colocalize in vesicular structures associated with the plastid surface, whereas the P450 proteins were detected in the endoplasmic reticulum. Whether they were expressed in Saccharomyces cerevisiae or in N. benthamiana, the TPS enzymes formed two different enantiomers of linalool: (—)-(R)-linalool for TPS10 and (+)-(S)-linalool for TPS14. Both P450 enzymes metabolize the two linalool enantiomers to form different but overlapping sets of hydroxylated or epoxidized products. These oxygenated products are not emitted into the floral headspace, but accumulate in floral tissues as further converted or conjugated metabolites. This work reveals complex linalool metabolism in Arabidopsis flowers, the ecological role of which remains to be determined.
Item Description: Copyright: © 2013 American Society of Plant Biologists
Physical Description: Online-Ressource
ISSN: 1040-4651

Similar Items

Cannot find similar records

Library Services

Search Options

Quick links

Orientation