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Anatomy and Dynamics of a Supramolecular Membrane Protein Cluster

Most plasmalemmal proteins organize in submicrometer-sized clusters whose architecture and dynamics are still enigmatic. With syntaxin 1 as an example, we applied a combination of far-field optical nanoscopy, biochemistry, fluorescene recovery after photobleaching (FRAP) analysis, and simulations... Full description

1st Person: Sieber, Jochen J.
Additional Persons: Willig, Katrin I. verfasserin; Kutzner, Carsten verfasserin; Gerding-Reimers, Claas verfasserin; Harke, Benjamin verfasserin; Donnert, Gerald verfasserin; Rammner, Burkhard verfasserin; Eggeling, Christian verfasserin; Hell, Stefan W. verfasserin; Grubmüller, Helmut verfasserin; Lang, Thorsten verfasserin
Source: in Science Vol. 317, No. 5841 (2007), p. 1072-1076
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Type of Publication: Article
Language: English
Published: 2007
Keywords: research-article
Online: Volltext
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100 1 |a Sieber, Jochen J. 
245 1 0 |a Anatomy and Dynamics of a Supramolecular Membrane Protein Cluster  |h Elektronische Ressource 
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500 |a Copyright: Copyright 2007 American Association for the Advancement of Science 
520 |a Most plasmalemmal proteins organize in submicrometer-sized clusters whose architecture and dynamics are still enigmatic. With syntaxin 1 as an example, we applied a combination of far-field optical nanoscopy, biochemistry, fluorescene recovery after photobleaching (FRAP) analysis, and simulations to show that clustering can be explained by self-organization based on simple physical principles. On average, the syntaxin clusters exhibit a diameter of 50 to 60 nanometers and contain 75 densely crowded syntaxins that dynamically exchange with freely diffusing molecules. Self-association depends on weak homophilic protein-protein interactions. Simulations suggest that clustering immobilizes and conformationally constrains the molecules. Moreover, a balance between self-association and crowding-induced steric repulsions is sufficient to explain both the size and dynamics of syntaxin clusters and likely of many oligomerizing membrane proteins that form supramolecular structures. 
653 |a research-article 
700 1 |a Willig, Katrin I.  |e verfasserin  |4 aut 
700 1 |a Kutzner, Carsten  |e verfasserin  |4 aut 
700 1 |a Gerding-Reimers, Claas  |e verfasserin  |4 aut 
700 1 |a Harke, Benjamin  |e verfasserin  |4 aut 
700 1 |a Donnert, Gerald  |e verfasserin  |4 aut 
700 1 |a Rammner, Burkhard  |e verfasserin  |4 aut 
700 1 |a Eggeling, Christian  |e verfasserin  |4 aut 
700 1 |a Hell, Stefan W.  |e verfasserin  |4 aut 
700 1 |a Grubmüller, Helmut  |e verfasserin  |4 aut 
700 1 |a Lang, Thorsten  |e verfasserin  |4 aut 
773 0 8 |i in  |t Science  |g Vol. 317, No. 5841 (2007), p. 1072-1076  |q 317:5841<1072-1076  |w (DE-601)JST078179041  |x 1095-9203 
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951 |a AR 
952 |d 317  |j 2007  |e 5841  |h 1072-1076 

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