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Construction of Large DNA Segments in Escherichia coli

Recombinant DNA clones containing large pieces of DNA are useful in the study of large genetic units, but these are difficult to make in most bacterial cloning vectors. A strategy is described that uses general and site-specific recombination to construct large pieces of eukaryotic DNA from... Full description

1st Person: O'Connor, Michael
Additional Persons: Peifer, Mark verfasserin; Bender, Welcome verfasserin
Source: in Science Vol. 244, No. 4910 (1989), p. 1307-1312
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Type of Publication: Article
Language: English
Published: 1989
Keywords: research-article
Online: Volltext
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