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Correction of CD18-Deficient Lymphocytes by Retrovirus-Mediated Gene Transfer

Leukocyte adhesion deficiency (LAD) is an inherited disorder of leukocyte function caused by derangements in CD 18 expression. The genetic and functional abnormalities in a lymphocyte cell line from a patient with LAD have been corrected by retrovirusmediated transduction of a functional CD18... Full description

1st Person: Wilson, James M.
Additional Persons: Ping, April Joy verfasserin; Krauss, John C. verfasserin; Mayo-Bond, Laura verfasserin; Rogers, Clare E. verfasserin; Anderson, Donald C. verfasserin; Todd, Robert F. verfasserin
Source: in Science Vol. 248, No. 4961 (1990), p. 1413-1416
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Type of Publication: Article
Language: English
Published: 1990
Keywords: research-article
Online: Volltext
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Summary: Leukocyte adhesion deficiency (LAD) is an inherited disorder of leukocyte function caused by derangements in CD 18 expression. The genetic and functional abnormalities in a lymphocyte cell line from a patient with LAD have been corrected by retrovirusmediated transduction of a functional CD18 gene. Lymphocytes from patients with LAD were exposed to CD 18-expressing retrovirus and enriched for cells that express CDlla and CD18 (LFA-1) on the cell surface. Molecular and functional analyses of these cells revealed (i) one copy of proviral sequence per cell, (ii) viral-directed CD18 RNA that exceeded normal endogenous levels, (iii) normal quantities of CD11a and CD18 protein on the cell surface, and (iv) reconstitution of LFA-l-dependent adhesive function.
Item Description: Copyright: Copyright 1990 American Association for the Advancement of Science
Physical Description: Online-Ressource
ISSN: 1095-9203

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