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Expression of the Nodulation Gene nodA in Rhizobium meliloti and Localization of the Gene Product in the Cytosol

The nodA gene of Rhizobium meliloti encodes a 21.8-kDa protein, which is conserved in several Rhizobium species. We overproduced the nodA protein as a fusion product with a portion of the λ cI repressor in Escherichia coli. This fusion protein was purified from inclusion bodies by gel and... Full description

1st Person: Schmidt, Jürgen
Additional Persons: John, Michael verfasserin; Wieneke, Ursula verfasserin; Krüssmann, Heinz-Dieter verfasserin; Schell, Jeff verfasserin
Source: in Proceedings of the National Academy of Sciences of the United States of America Vol. 83, No. 24 (1986), p. 9581-9585
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Type of Publication: Article
Language: English
Published: 1986
Keywords: research-article
Genetics
Fusion Protein
Antibodies
Nodules
Online: Volltext
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