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Determination of the Transiently Lowered pKaof the Retinal Schiff Base during the Photocycle of Bacteriorhodopsin

Reprotonation of the transiently deprotonated retinal Schiff base in the bacteriorhodopsin photocycle is greatly slowed when the proton donor Asp-96 is removed with site-specific mutagenesis, but its rate is restored upon adding azide or other weak acids such as formate and cyanate. As expected,... Full description

1st Person: Brown, Leonid S.
Additional Persons: Lanyi, Janos K. verfasserin
Source: in Proceedings of the National Academy of Sciences of the United States of America Vol. 93, No. 4 (1996), p. 1731-1734
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Type of Publication: Article
Language: English
Published: 1996
Keywords: research-article
Biophysics
Online: Volltext
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